ACAD9 Antibody 15770-1-AP | Proteintech

来自 : 发布时间：2024-12-25

蛋白质印迹 ppt

我们的蛋白质印迹技术指南的幻灯片概述。

跳转至：Western Blot 实验方案如何优化您的 Western BlotSDS-PAGE 凝胶配方如何使用低分子量蛋白质优化您的结果低分子量蛋白的 Tricine 凝胶配方选择正确的裂解缓冲液选择正确的 Western Blot 检测方法 Western Blot 故障排除：为什么观察到的蛋白质分子量 (MW) 与计算值不同？蛋白质印迹疑难解答：高背景蛋白质印迹疑难解答：弱/无信号和其他蛋白质印迹 ppt 蛋白质印迹视频协议下载 PDF 版本蛋白质印迹 - 技术提示和疑难解答

蛋白质印迹- Proteintech Group 的技术提示和故障排除用于低分子量蛋白质的 Tricine 凝胶配方

用于蛋白质印迹的 Tricine SDS-PAGE 凝胶配方，适用于小于 20 kDa 的目标蛋白。

跳转至：Western Blot 实验方案如何优化您的 Western BlotSDS-PAGE 凝胶配方如何使用低分子量蛋白质优化您的结果低分子量蛋白的 Tricine 凝胶配方选择正确的裂解缓冲液选择正确的 Western Blot 检测方法 Western Blot 故障排除：为什么观察到的蛋白质分子量 (MW) 与计算值不同？Western Blot 疑难解答：高背景Western Blot 疑难解答：弱/无信号和其他Western Blot pptWestern Blot 视频协议下载 PDF 版本

对于 MW 小于 20 kDa 的目标蛋白，a Tricine 凝胶系统可以获得更高的分辨率，强烈推荐。如下表和图表所示，制作三层 Tricine 凝胶。将特定的 Tricine 凝胶电泳缓冲液加入到运行系统中并照常进行转移al.

配方 1物品堆叠中级分离凝胶百分比4%10%15%凝胶体积2ml3ml6ml38%甘油--1.6ddH201.41.2-30%丙烯酰胺0.30.82.73.0 M Tris HCl (pH 8.5)-12.141.0 M Tris HCl (pH 6.8)0.3- -10% SDS0.020.030.0610% APS0.020.030.06TEMED0.0020.0030.003

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Proteintech 抗体对您不想减掉的脂肪的新发现

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两篇自然论文揭示了更多细节棕色和米色脂肪组织的作用。

棕色脂肪组织是一种脂肪，通过\"非颤抖产热”帮助提高体温。棕色脂肪的激活因其高代谢能力而引起研究人员的极大兴趣，可用于治疗肥胖相关疾病。长时间的寒冷暴露还会导致白色脂肪细胞变成棕色或米色，并采用代谢更活跃的棕色样表型。

Seiki 等人最近在《自然》杂志上发表的一篇文章。发现通过寒冷激活棕色脂肪可以通过干扰癌细胞中基于糖酵解的代谢来抑制肿瘤生长。这项研究提供了一种新的范例作为其他癌症疗法的辅助手段。在另一篇《自然》杂志上呃，王等人。发现了米色脂肪如何通过 PRDM16 激活，以及增加其翻译后稳定性如何抵消小鼠饮食引起的肥胖。这些论文在其研究中总共引用了 9 种 Proteintech 抗体。

 

使用 23673-1-AP（UCP1 抗体）稀释 1:4000（下）对小鼠棕色脂肪组织载玻片进行 IHC 分析40 倍镜头）。使用 Tris-EDTA 缓冲液 (pH 9.0) 进行热介导抗原修复。

 

棕色脂肪研究的热门抗体：

UCP1

Glut1

Glut4

胰岛素

ATP5A1

MCT02

 

 

1 个结果：\"活性染料”

过滤器

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应用说明染色还是不染色？了解可用于流式细胞术的不同活性染料

了解可用于帮助您在流式细胞术中获得可靠、准确结果的活性染料类型。

Validation Data GalleryWB analysis using 15770-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 15770-1-AP (ACAD9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.View All Images (4)×Various lysates were subjected to SDS PAGE followed by western blot with 15770-1-AP (ACAD9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC staining of human liver cancer using 15770-1-APImmunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (4)×Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human liver cancer using 15770-1-APImmunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (4)×Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). -/ - Cat No. 15770-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHEK-293T cells, HEK-293 cells, MCF-7 cellsPositive IHC detected inhuman liver cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:1000Immunohistochemistry (IHC)IHC : 1:50-1:500Sample-dependent, check data in validation data gallery 15770-1-AP targets ACAD9 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples. Calculated molecular weight 69 kDaObserved molecular weight 65 kDaGenBank accession numberBC007970 Gene symbol ACAD9 Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationAcyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency). ProtocolsProduct Specific ProtocolsWB protocol for ACAD9 antibody 15770-1-APDownload protocolIHC protocol for ACAD9 antibody 15770-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (3)KD/KO (1)WB (3)SpeciesApplicationTitlehumanWBCell RepA membrane arm of mitochondrial complex I sufficient to promote respirasome formation.Authors - Hezhi FangKD ValidatedView ArticlemouseWBSci RepIntegrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.Authors - David W ReidView ArticlehumanWBEMBO RepMITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.Authors - Cong WangView Article WB FiguresWB analysis of HEK-293 using 15770-1-APHEK-293 cells were subjected to SDS PAGE followed by western blot with 15770-1-AP (ACAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis using 15770-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 15770-1-AP (ACAD9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC FiguresIHC staining of human liver cancer using 15770-1-APImmunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human liver cancer using 15770-1-APImmunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). \"ACAD9 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare.