Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells,SH-SY5Y cells,PC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 88 publications below |
| IHC | See 1 publications below |
Product Information
51151-1-AP targets JNK inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.
| Tested Reactivity | human,mouse |
| Cited Reactivity | A. sphaerocephala Krasch, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | mitogen-activated protein kinase 8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 44-48 kDa, 50-55 kDa |
| GenBank accession number | NM_138982 |
| Gene symbol | MAPK8 |
| Gene ID (NCBI) | 5599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for-20oC storage. |
Background Information
Function
JNK1, also known as mitogen-activated protein kinase 8 – MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.
Tissue specificity
JNK1 is ubiquitously expressed.
Involvement in disease
JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.
Isoforms
There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.
Post-translational modifications
JNK1 is phosphorylated by MAPK kinases (MAPKK).
Cellular localization
JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JNK antibody 51151-1-AP | Download protocol |
| IF protocol for JNK antibody 51151-1-AP | Download protocol |
| IP protocol for JNK antibody 51151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
