CDC25A Antibody 55031-1-AP | Proteintech

来自 : 发布时间：2024-12-25

职业现场营销代表，丹麦现场营销代表，丹麦

现场营销代表职位空缺

兼职（每月约 15 小时）临时合同有竞争力的薪酬基于您所在的机构介绍

Proteintech 是一家国际生物技术公司，生产用于生命科学研究的抗体、ELISA 试剂盒和蛋白质。 Proteintech 按照最高标准生产自己的所有产品，并完全控制整个制造、验证和质量控制流程。 Proteintech 产品的引用次数超过 170,000 次，这证明了对公司最重要的一点：其试剂在科学家手中取得成功，帮助他们做出改变和解释我们周围世界的发现。

新创建的角色是立足于该领域，专注于提高 Proteintech 的品牌知名度，并提高其在关键研究机构生命科学界的影响力。

这是一个理想的机会对于想要在营销和科学传播方面获得一些商业经验的博士生来说，这是一个很好的选择。

Proteintech 的商业营销计划为有抱负的博士生提供营销、科学传播和活动策划经验。该角色大约是。每月 15 小时，可以根据您想要获得的经验进行定制。 Proteintech 可以在完成该计划后为您的简历提供参考。

活动包括：

作为 Proteintech 现场营销代表，负责：

      帮助组织活动和虚拟活动机构。管理邀请和出席。通过传单、口碑、社交媒体和电子邮件提高 Proteintech 在研究所的品牌知名度。支持和促进机构内特定的蛋白质科技营销活动。促进大学实验室和 Proteintech 之间的沟通，提供反馈、想法和市场数据。撰写客座博客（当机会可用）

 理想候选人

      对科学传播或营销职业感兴趣。热情、外向，擅长建立合作和网络。候选人必须是英国机构生命科学专业的现任博士生。理想情况下，他们将参与相关的生命科学协会/俱乐部。定期使用抗体并了解抗体应用      良好的时间管理和组织能力。

 关键信息

      临时合同 - 6 或 12 个月的计划      兼职 - 每月约 15 小时      有竞争力的薪酬

 名额有限，将会根据非正式面试进行分配。如需申请，请将您的简历通过电子邮件发送至 rebecca@ptglab.com

职业现场营销代表，荷兰现场营销代表，荷兰

现场营销代表职位空缺

兼职（每月约 15 小时）临时合同有竞争力的薪酬基于您所在的机构简介

Proteintech 是一家国际生物技术公司，生产用于生命科学研究的抗体、ELISA 试剂盒和蛋白质。 Proteintech 按照最高标准生产自己的所有产品，并完全控制整个制造、验证和质量控制流程。 Proteintech 产品的引用次数超过 170,000 次，这证明了对公司最重要的一点：其试剂在科学家手中取得成功，帮助他们做出改变和解释我们周围世界的发现。

新创建的角色是立足于该领域，专注于提高 Proteintech 的品牌知名度，并提高其在关键研究机构生命科学界的影响力。

这是一个理想的选择为想要在营销和科学传播方面获得一些商业经验的博士生提供了机会。

Proteintech 的商业营销计划为有抱负的博士生提供营销、科学传播和活动策划经验。该角色大约是。每月 15 小时，可以根据您想要获得的经验进行定制。 Proteintech 可以在完成该计划后为您的简历提供参考。

活动包括：

作为 Proteintech 现场营销代表，负责：

      帮助组织活动和虚拟活动机构。管理邀请和出席。通过传单、口碑、社交媒体和电子邮件提高 Proteintech 在研究所的品牌知名度。支持和促进机构内特定的蛋白质科技营销活动。促进大学实验室和 Proteintech 之间的沟通，提供反馈、想法和市场数据。撰写客座博客（什么有机会）

 理想候选人

      对科学传播或营销职业感兴趣。热情、外向，擅长建立合作和网络。候选人必须是英国机构生命科学专业的现任博士生。理想情况下，他们将参与相关的生命科学协会/俱乐部。定期使用抗体并了解抗体应用      良好的时间管理和组织能力。

 关键信息

      临时合同 - 6 或 12 个月的计划      兼职 - 每月约 15 小时      有竞争力的薪酬

 名额有限，将会根据非正式面试进行分配。如需申请，请将您的简历通过电子邮件发送至 rebecca@ptglab.com

Validation Data GalleryWB analysis of HepG2 using 55031-1-APHepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.View All Images (10)×HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (10)×Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (10)×Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF Staining of human breast cancer using 55031-1-APImmunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).View All Images (10)×Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP experiment of HeLa using 55031-1-APIP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.View All Images (10)×IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug. -/ - Cat No. 55031-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHepG2 cells, mouse liver tissuePositive IP detected inHeLa cellsPositive IHC detected inhuman breast cancer tissue, human gliomas tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inhuman breast cancer tissue Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:3000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data gallery 55031-1-AP targets CDC25A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples. Calculated molecular weight 59 kDaObserved molecular weight 60 kDaGenBank accession numberNM_001789 Gene symbol CDC25A Background InformationCDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A. ProtocolsProduct Specific ProtocolsWB protocol for CDC25A antibody 55031-1-APDownload protocolIHC protocol for CDC25A antibody 55031-1-APDownload protocolIF protocol for CDC25A antibody 55031-1-APDownload protocolIP protocol for CDC25A antibody 55031-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (19)KD/KO (1)WB (18)IHC (5)IF (4)SpeciesApplicationTitlehumanWBAm J Transl ResDecreased expression of miR-184 restrains the growth and invasion of endometrial carcinoma cells through CDC25A-dependent Notch signaling pathway.Authors - Zhen ChenKD ValidatedView ArticlehumanWBCell CycleA novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.Authors - Yanan WangView ArticlehumanWBCell CyclePMID: 31599189Authors - Yonglong ZhangView ArticlehumanWB, IFInt J Mol SciUbiquitin-Specific Protease 29 Regulates Cdc25A-Mediated Tumorigenesis.Authors - Arun Pandian ChandrasekaranView ArticlehumanWB,IHCAm J Transl ResInhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.Authors - Tao LiuView ArticlehumanIFEur J PharmacolHypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.Authors - Jinhang HuView Article See more citations WB FiguresWB analysis of HepG2 using 55031-1-APHepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.WB analysis of mouse liver using 55031-1-APmouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC FiguresIHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).IHC staining of human breast cancer using 55031-1-APImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).IHC staining of human gliomas using 55031-1-APImmunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).IHC staining of human gliomas using 55031-1-APImmunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).IP FiguresIP experiment of HeLa using 55031-1-APIP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.IF FiguresIF Staining of human breast cancer using 55031-1-APImmunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). \"CDC25A antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:500-1:3000IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WBIHC : 1:50-1:500IF : 1:50-1:500