Endoglin/CD105 Antibody 10862-1-AP | Proteintech

来自 : 发布时间：2024-12-25

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×搜索还提供标准实验方案目录号产品名称特定实验方案查看更多蛋白质科技 StoryView更新资料更新送货地址更新帐单地址发票ID订单号客户发票日期总计尚无商品。加载更多加载更多订单号订单日期客户总计状态尚无商品。加载更多加载更多报价编号客户报价日期到期日期TotalStatus尚无项目。加载更多加载更多未返回搜索结果 很遗憾，搜索引擎无法找到与您的搜索词相匹配的产品。请填写产品查询表，我们的团队将尽力为您找到相关产品。购买概述产品信息出版物 (4)HRP 标记的 Affinipure 山羊抗豚鼠 IgG(H+L)

应用

ELISA、WB

结合

HRP

宿主

山羊

反应性

豚鼠

出版物

4

目录号：SA00001-12

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概述

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产品信息出版物 (4)

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打印数据表

MSDS

产品信息产品信息应用ELISA、WB宿主物种山羊物种反应性豚鼠物理状态液体建议稀释范围 1:2000-1:10,000 用于 ECL 底物的蛋白质印迹 1:1000-1:20,000 用于 ELISA 和蛋白质印迹显色底物纯度使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中纯化抗体。储存缓冲液0.01 M 磷酸钠，0.25 M NaCl，50% 甘油，3 mg/ml BSA，pH 7.6。保存于-20°C。发货后稳定一年。稳定剂3 mg/ml BSARRIDAB_2890975注意本产品仅供研究使用，不可用于诊断或治疗用途。 PublicationsTitle

J Biol Chem

ER 进入途径和 GPI 锚定蛋白的糖基化由 N 端信号序列和 C 端 GPI 连接序列决定

未返回搜索结果 很遗憾，搜索引擎无法找到与您的搜索词相匹配的产品。请填写产品查询表，我们的团队将尽力为您找到相关产品。多重分析

使用 FlexAble 抗体标记试剂盒进行多重分析的注意事项

跳转至：一般说明标准工作流程多重分析常见问题步骤 1-4 参见\"标准工作流程”

 

不同物种的一抗可以在同一管中组合，并使用相应的物种特异性 FlexLinkers 同时标记。根据要标记的一抗的量适当扩大试剂体积。    

 

对于同一物种的一抗，单独标记每个抗体。标记一抗后，将其组合并用于您所需的应用。

    

   

用 FlexAble 试剂盒标记的抗体可与预缀合一抗一起使用.

 

下载 PDF 格式的协议

标准工作流程

标记 0.5μg 一抗  

跳转至：一般说明标准工作流程多重处理常见问题

 

开始之前，将所有试剂平衡至室温

   程序：将 0.5 µg 一抗与 1 µL FlexLinker 混合。添加 FlexBuffer，使总体积达到 8μL。轻轻混匀，室温避光孵育 5 分钟。加入 2μL FlexQuencher。轻轻混匀，室温避光孵育 5 分钟。抗体即可使用。按比例放大以标记更多数量的一抗

 

下载 PDF 格式的实验方案

Endoglin/CD105 Polyclonal antibody 7 Publications Rabbit Polyclonal| Catalog number: 10862-1-AP IHC, IP, WB,ELISA Application not tested? Please see our Guarantee Images See allVarious lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP Result of anti-Endoglin/CD105 (IP:10862-1-AP, 3ug; Detection:10862-1-AP 1:300) with mouse lung tissue lysate 4000ug. Positive WB detected in:human placenta tissuePositive IP detected in:mouse lung tissuePositive IHC detected in:human breast cancer tissue, human placenta tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Recommended dilution:WB : 1:500-1:1000IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WBIHC : 1:1000-1:4000 Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. BackgroundEndoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481) Author:X-H ZhangPubmed ID:29565476Journal:Eur Rev Med Pharmacol SciTitle:MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.Application:WB,IHC Species:humanAuthor:Xin-Xin ZhaoPubmed ID:30460778Journal:J Exp Zool B Mol Dev EvolTitle:Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.Application:IF Species:BovineAuthor:Qi LiPubmed ID:32125888Journal:Cell ReprogramTitle:Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.Application: Species:Author:Rina Fujii-TezukaPubmed ID:33426237Journal:Regen TherTitle:Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.Application:IF Species:humanAuthor:Graham N NPubmed ID:22401877Journal:PlacentaTitle:Placental expression of anti-angiogenic proteins in mirror syndrome: a case report.Application:IHC Species:humanAuthor:Kakigano A APubmed ID:23611482Journal:PlacentaTitle:Imbalance of angiogenic factors and avascular edematous cystic villi in a trisomy 13 pregnancy: a case report.Application:IHC Species:human Load More WB FiguresVarious lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC FiguresImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP FiguresIP Result of anti-Endoglin/CD105 (IP:10862-1-AP, 3ug; Detection:10862-1-AP 1:300) with mouse lung tissue lysate 4000ug.