VDAC1/2 Antibody 10866-1-AP | Proteintech

来自 : 发布时间：2024-12-25

公司新闻重要通知：欧洲客户临时传真号码重要通知：欧洲客户临时传真号码

Proteintech Europe 目前客户传真线路遇到技术困难。

请使用 +441612413103下达任何传真订单，直至另行通知。

目前，我们无法接收使用 +44 161 23 21 272 下达的任何传真订单。请将 9 月 2 日之后下达的所有订单重新发送至我们的临时传真号码。对于给您带来的任何不便，我们深表歉意，并感谢您的耐心等待。如果您想检查现有订单的状态或需要进一步帮助，请通过电子邮件联系我们：europe@ptglab.com。

请注意，此次中断不会影响我们的美国传真号码。

>公司新闻新公司视频新公司视频

了解 Proteintech 抗体如何加速您的研究

---

Proteintech：科学家提供的抗体

查找通过观看此视频，了解 Proteintech 抗体如何帮助我们的客户加速他们的研究。

 

作为一家由科学家创立的公司，Proteintech Group 认可我们的不懈努力以及研究人员为推进科学发现而做出的牺牲。

Proteintech 旨在通过提供高质量、可靠的试剂来实现可重复的结果，从而满足客户对研究的奉献精神。

公司新闻公布 AACR 2017 旅行补助金公布 AACR 2017 旅行补助金

Proteintech Group 再次赞助两项 1,000 美元的择优奖学金，以参加 #AACR17

由 CG Life 为 Proteintech Group 撰写< /p>

芝加哥 - 2017 年 2 月 20 日 - 抗体标杆 Proteintech Group 今天宣布启动 2017 年美国癌症研究协会 (AACR) 会议的年度旅行补助竞赛。两位获奖者将各获得 1,000 美元的补助金，用于本次会议的住宿和旅行。 AACR 2017 将于 4 月 1 日至 5 日在华盛顿特区举行。获奖者可以用这笔钱支付活动注册费、机票和住宿费。

旅行奖向大学或研究机构的所有癌症研究人员开放机构。申请要求研究人员向官方竞赛网站提交当前研究的摘要或其在 AACR 2017 上提交的摘要副本。今年新推出独立t，选出获奖者的国际小组完全由博士后研究员和研究生组成。

\"我们需要投资于早期职业研究人员，”Proteintech 首席执行官 Jason Li 博士说。 \"这意味着为他们提供充足的机会和挑战来成长为科学家。我们高素质的专家组将奖励最具原创性和最有价值的癌症研究。”

每年春季，AACR 年会都会吸引来自 60 多个国家的 18,000 多名与会者，其中包括 2016 年获奖者 Ariella Hanker。

\"我在 AACR 建立了几位新联系人，现在正在与他们合作，”范德比尔特大学医学研究讲师汉克说。 \"离开 AACR 时，我总是对我的研究感到充满活力，并对我们在抗击癌症方面取得的进展感到乐观。”

只有在 3 月 6 日截止日期之前提交的前 200 份参赛作品才会被接受，并且获奖者将于 2017 年 3 月 13 日公布。AACR 制定的所有禁运政策将受到尊重。

要申请 #AACR17 的 Proteintech 旅行补助金，或了解官方规则、条款和条件，请访问 bit.ly/2l5zAgl。

 

公司新闻欧洲客户：重要通知欧洲客户：重要通知

欧洲客户重要通知

---

请注意我们的电子邮件地址 Europe@ptglab.com 和Germany@ptglab。 com 从 2 月 24 日星期五下午到 2 月 27 日星期一上午处于离线状态。

如果您在此期间发送订单或查询，我们将不会收到它们。请立即将任何查询/订单重新发送到相同的地址，以确保它们得到处理。

对于由此给您带来的任何不便，我们深表歉意。

Synonyms hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC2 Proteintech vs. Other VendorsValidation Data Gallery\"VDAC1/2 Antibodies\" ComparisonView side-by-side comparison of VDAC1/2 antibodies from other vendors to find the one that best suits your research needs.Compare nowWB analysis using 10866-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.View All Images (20)×Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.IP experiment of mouse skeletal muscle using 10866-1-APIP Result of anti-VDAC1 (IP:10866-1-AP, 3ug; Detection:10866-1-AP 1:1000) with mouse skeletal muscle tissue lysate 7500ug.View All Images (20)×IP Result of anti-VDAC1 (IP:10866-1-AP, 3ug; Detection:10866-1-AP 1:1000) with mouse skeletal muscle tissue lysate 7500ug.IHC staining of human colon using 10866-1-APImmunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).View All Images (20)×Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).IHC staining of human colon using 10866-1-APImmunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).View All Images (20)×Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).IF Staining of human liver cancer using 10866-1-APImmunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).View All Images (20)×Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF Staining of human liver cancer using 10866-1-APImmunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).View All Images (20)×Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). -/ - Cat No. 10866-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHEK-293 cells, 4T1 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, MCF-7 cells, MDA-MB-453s cells, rat heart tissue, rat kidney tissuePositive IP detected inmouse skeletal muscle tissuePositive IHC detected inhuman colon tissue, human pancreas tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inhuman liver cancer tissue Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:3000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data gallery 10866-1-AP targets VDAC1/2 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples. Calculated molecular weight 31 kDaObserved molecular weight 31 kDaGenBank accession numberBC008482 Gene symbol VDAC1 Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationVDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2. ProtocolsProduct Specific ProtocolsWB protocol for VDAC1/2 antibody 10866-1-APDownload protocolIHC protocol for VDAC1/2 antibody 10866-1-APDownload protocolIF protocol for VDAC1/2 antibody 10866-1-APDownload protocolIP protocol for VDAC1/2 antibody 10866-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (78)KD/KO (2)WB (72)IHC (2)IF (6)IP (2)CoIP (1)SpeciesApplicationTitlehumanWBEBioMedicineTargeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.Authors - Binghua LiView ArticlehumanWBHum Mol GenetContribution of mitochondrial ND1 3394T>C mutation to the phenotypic manifestation of Leber\'s hereditary optic neuropathy.Authors - Yanchun JiView ArticlemouseWBTheranosticsp66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species.Authors - Yan ZhaoView ArticlezebrafishNucleic Acids ResDeletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.Authors - Danni ChenView ArticlehumanWBJ ImmunolThe Otubain YOD1 Suppresses Aggregation and Activation of the Signaling Adaptor MAVS through Lys63-Linked Deubiquitination.Authors - Chang LiuView ArticlehumanWBCell CycleA novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.Authors - Yanan WangView Article See more citations ReviewsAdd a reviewThe reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.FHAlberto (Verified Customer) (01-09-2020)Clear sharp band at the expected molecular weight.Applications: Western Blot,Primary Antibody Dilution: 1:1000Cell Tissue Type: Mouse brainVDAC1/2 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain (Cat no:10866-1-AP)FHWanxia (Verified Customer) (10-22-2019)Excellent WB results and very low background.Applications: Western Blot,Primary Antibody Dilution: 1:500 dilutionCell Tissue Type: Mouse Brain Related ProductsBcl-XL Rabbit Polyclonal antibody10783-1-APBcl-XL Mouse Monoclonal antibody66020-1-IgBAX Rabbit Polyclonal antibody50599-2-IgBCL2 Rabbit Polyclonal antibody12789-1-APCOXIV Rabbit Polyclonal antibody11242-1-APCytochrome c Rabbit Polyclonal antibody10993-1-APVDAC2 Rabbit Polyclonal antibody11663-1-APVDAC3 Rabbit Polyclonal antibody14451-1-AP WB FiguresWB analysis using 10866-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB analysis of DU 145 using 10866-1-APDU 145 cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis using 10866-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.WB analysis using 10866-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.WB analysis of HEK-293 using 10866-1-APHEK-293 cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of HeLa using 10866-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of HL-60 using 10866-1-APHL-60 cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of human heart using 10866-1-APhuman heart tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of Jurkat using 10866-1-APJurkat cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of mouse brain using 10866-1-APmouse brain tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of mouse heart using 10866-1-APmouse heart tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of mouse kidney using 10866-1-APmouse kidney tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis using 10866-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC FiguresIHC staining of human colon using 10866-1-APImmunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).IHC staining of human colon using 10866-1-APImmunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).IHC staining of human pancreas using 10866-1-APImmunohistochemical analysis of paraffin-embedded human pancreas using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).IHC staining of human pancreas using 10866-1-APImmunohistochemical analysis of paraffin-embedded human pancreas using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).IP FiguresIP experiment of mouse skeletal muscle using 10866-1-APIP Result of anti-VDAC1 (IP:10866-1-AP, 3ug; Detection:10866-1-AP 1:1000) with mouse skeletal muscle tissue lysate 7500ug.IF FiguresIF Staining of human liver cancer using 10866-1-APImmunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF Staining of human liver cancer using 10866-1-APImmunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). \"VDAC1/2 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:500-1:3000IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WBIHC : 1:50-1:500IF : 1:50-1:500