Tested Applications
Positive WB detected in | rat testis tissue, NCCIT cells,Neuro-2a cells,L02 cells,pig cerebellum tissue,rat brain tissue,mouse ovary tissue,HEK-293 cells,mouse testis |
Positive IHC detected in | mouse ovary tissue, human gliomas tissue,rat brain tissue,mouse brain tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 20 publications below |
IF | See 16 publications below |
Product Information
66200-1-Ig targets acetylated Tubulin(Lys40) inWB, IHC, IF, ELISA applications and shows reactivity with human,mouse,rat,dog, pig samples.
Tested Reactivity | human,mouse,rat,dog, pig |
Cited Reactivity | bovine, Drosophila, human, mouse, rat, swine |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | tubulin, alpha 1a |
Calculated molecular weight | 52 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | NM_006009 |
Gene symbol | TUBA1A |
Gene ID (NCBI) | 7846 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
What is the molecular weight of acetyl-α-tubulin?
The molecular weight of acetylated tubulin is 52 kD.
Where does the acetylation of Lys 40 occur?
This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).
How can acetylation be reversed?
Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).
What is the function of acetylation?
Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).
Is acetylation of α-tubulin strictly associated with stable microtubules?
Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).
Is ac-tubulin found only in cilia?
Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).
What are the cellular effects of tubulin acetylation?
Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
IHC protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
IF protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |