CD134/OX40 Antibody 20006-1-AP | Proteintech

来自 : 发布时间：2024-12-25

主页E.大肠杆菌表达的融合蛋白E．大肠杆菌表达的融合蛋白

融合蛋白是许多实验室技术的有用工具，例如蛋白质纯化或封闭实验。 Proteintech 提供多种高质量融合蛋白。

我们的客户服务提供融合蛋白和肽文库，可用作细胞测定中的标准品和对照和免疫测定（请注意，我们的融合蛋白未经活性测试，不适合体内研究）。

查看所有融合蛋白相关内容博客蛋白质的复杂性

如何蛋白质仅基于 20 个构建模块就具有如此多的功能多样性？

非可见文本博客肽与蛋白质免疫原

蛋白质和肽免疫原的优点和缺点。

不可见文本ProductcGMP 级细胞因子和生长因子

用于细胞和基因治疗。

N可见文本博客如何选择重组蛋白

选择细胞因子和生长因子时的 4 个注意事项。

不可见文本博客博客蛋白质的复杂性

蛋白质如何仅基于 20 个构建模块就具有如此多的功能多样性？

非可见文本博客博客肽与蛋白质免疫原

蛋白质和肽免疫原的优点和缺点。

不可见文本ProductProductcGMP 级细胞因子和生长因子

用于细胞和基因治疗。

不可见文本BadgeBadge如何选择重组蛋白

选择细胞因子和生长因子时的 4 个注意事项。

不可见文本HomeChromoTek 基于纳米抗体的试剂纳米抗体 / VHH纳米抗体 / VHH

未缀合的初级单域抗体

VHH，也称为纳米抗体，源自骆驼科动物的仅重链抗体。 ChromoTek 的 VHH 经过充分表征和验证。由于这些单域抗体 (sdAb) 的分子量仅为 15 kDa 左右，因此 VHH 的大小仅为传统 IgG 抗体 (150 kDa) 的十分之一，或者不到 Fab 片段 (50 kDa) 大小的三分之一)。

特性：

小尺寸单肽链结构

应用：

适合通过NHS酯反应与染料、生物素、珠子、表面等缀合探索纳米抗体/ VHHs探索纳米抗体 / VHHs

GFP-GFP纳米抗体com的结构plex.

ProductFormats（括号中的产品引用）GFP VHHUnconjuated (23)GFP VHH、生物素化BiotinylatedGST VHHUnconjuated (9)Halo VHHUnconjueratedHistone VHHUnconjuatedMBP VHHUnconjuatedMK2 VHHUnconjueratedmNeonGreen VHHUnconjuatedMyc VHHUnconjueratedp53 N-term VHHUn结合的 p53 C 端 VHH 未结合的 PARP1 VHH 未结合的 RFP VHH 未结合的(4)SNAP/CLIP- tag® VHHUnconjuatedSpot VHHUnconjuated(1)TurboGFP VHHUnconjuatedV5 VHHUnconjuatedVimentin VHHUnconjuatedHomeChromoTek 基于纳米抗体的试剂Nano-CapsNano-Caps

蛋白质纯化树脂

Nano-Caps 是一种优质亲和树脂，针对有效且经济的一步蛋白质纯化进行了优化。它们由与琼脂糖珠缀合的纳米抗体/ VHH 组成。

优点：

在 4°C 下快速一步纯化蛋白质高结合容量高效肽洗脱频繁再生可多次重复使用高特异性

应用：蛋白质纯化探索纳米胶囊探索纳米胶囊< p class=\"small text-center\">使用 Spot-Cap 纯化点标记 GFP。

ProductFormatSpot-CapAgarose 珠子主要目录

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用于癌症研究的抗体

用于神经科学研究的抗体

使用纳米抗体获得更好的图像

流式细胞术

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用于卓越免疫沉淀的纳米陷阱

用于心血管研究的抗体

用于免疫反应的抗体

用于神经回路的抗体

神经相关疾病的抗体

免疫细胞标记物

技术指南

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免疫沉淀：概述和技术提示

裂解液制备：技术提示

细胞培养完整指南

流式细胞术完整指南

免疫组织化学实验完整指南

完整指南优化免疫荧光染色

蛋白质印迹完整指南

产品重点

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自噬研究重点

细胞骨架标记

神经标记物抗体

二抗

去泛素化酶

乳腺癌焦点

肾癌cer Focus

外泌体标记

CD标记抗体

线粒体标记

表观遗传学抗体

白皮书

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BMP2 - HumanKine 优势

IL-2 - HumanKine 优势

Wnt3a - HumanKine 优势

FGF 基本耐热

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Synonyms ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L Validation Data GalleryWB analysis of HepG2 using 20006-1-APHepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.View All Images (4)×HepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.FC experiment of U-937 using 20006-1-AP1X10^6 U-937 cells were stained with 0.2ug CD134/OX40 antibody (20006-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.View All Images (4)×1X10^6 U-937 cells were stained with 0.2ug CD134/OX40 antibody (20006-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. -/ - Cat No. 20006-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHepG2 cells, L02 cells, U-937 cellsPositive FC detected inU-937 cells Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:1000Sample-dependent, check data in validation data gallery 20006-1-AP targets CD134/OX40 in WB, FC,ELISA applications and shows reactivity with human samples. Calculated molecular weight 29 kDaObserved molecular weight 43 kDaGenBank accession numberNM_003327 Gene symbol TNFRSF4 Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationCD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5. ProtocolsProduct Specific ProtocolsWB protocol for CD134/OX40 antibody 20006-1-APDownload protocolFC protocol for CD134/OX40 antibody 20006-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols WB FiguresWB analysis of HepG2 using 20006-1-APHepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.WB analysis of L02 using 20006-1-APL02 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.WB analysis of U-937 using 20006-1-APU-937 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.FC FiguresFC experiment of U-937 using 20006-1-AP1X10^6 U-937 cells were stained with 0.2ug CD134/OX40 antibody (20006-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. \"CD134/OX40 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare.