Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells,C2C12 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human endometrial cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 66 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
14052-1-AP targets CDK6 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDK6 fusion protein Ag5275 |
| Full Name | cyclin-dependent kinase 6 |
| Calculated molecular weight | 326 aa, 36 kDa |
| Observed molecular weight | 36-40 kDa |
| GenBank accession number | BC027989 |
| Gene symbol | CDK6 |
| Gene ID (NCBI) | 1021 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Cyclin-dependentkinase6(CDK6)isanenzymebelongingtotheCDKfamily.CDK6isacatalyticsubunitofa
proteinkinasecomplexthatisessentialforprogressionthroughtheG1phaseofthecellcycleandG1/Stransition,
thusactivatingcellproliferation.CDK6partnerswithcyclinstophosphorylatetheretinoblastoma(pRb)proteinto
releaseitsbindingpartnerE2F;atranscriptionfactorthatdrivesgeneexpressionnecessaryforDNAreplication
(PMID:8114739).CDK6waslongthoughtofasaredundanthomologofCDK4astheyperformsimilarrolesincell
cycleprogression.ButrecentlyithasbecomeapparentthatCDK6andCDK4differintissue-specificfunctions,
whereCDK6playsaroleindifferentiation(PMID:16410727)butalsoincontributiontotumordevelopment.
WhatisthemolecularweightofCDK6?
ThemolecularweightofCDK6isapproximately36–40kDa(PMID:23563707,8114739).
WhatisthesubcellularlocalizationofCDK6?
CDK6isubiquitouslyexpressedthroughthenucleusandcytoplasm.However,themostactivecomplexesare
locatedinthenucleiofproliferatingcells.
WhatistheexpressionpatternofCDK6?
CDK6activityfirstoccursmidwaythroughtheG1phase.ItsexpressionisregulatedbyD-typecyclinsand
membersoftheINK4familyofCDKinhibitors(PMID:7739547).
WhatisCDK6’sroleincancer?
CDK6isinvolvedinacriticalregulatorypointinthecellcycleandsoisfoundtobeunbalancedinapproximately
80–90%oftumors(PMID:23356980).Lossofcellcyclecontrolistheinitialstepinthedevelopmentofthe
hallmarksofcancer,suchassustainedproliferativesignalinganddysregulatedcellularenergetics.Deregulation
ofCDK6hasanimportantroleinlymphomabyincreasingangiogenesis(PMID:23948297)andupregulatedCDK6
expressionisalsoassociatedwithapoorclinicalprognosisinmedulloblastoma(PMID:23172372).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK6 antibody 14052-1-AP | Download protocol |
| IHC protocol for CDK6 antibody 14052-1-AP | Download protocol |
| IF protocol for CDK6 antibody 14052-1-AP | Download protocol |
| IP protocol for CDK6 antibody 14052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
