ProteinTech/CDK4多克隆抗体/150ul/11026-1-AP
商品编号：
11026-1-AP
品牌：
ptglab
市场价：
¥1980.00
美元价：
1188.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells,rat pancreas tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 130 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
11026-1-AP targets CDK4 inWB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | canine, human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDK4 fusion protein Ag1505 |
| Full Name | cyclin-dependent kinase 4 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC010153 |
| Gene symbol | CDK4 |
| Gene ID (NCBI) | 1019 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK4 antibody 11026-1-AP | Download protocol |
| IHC protocol for CDK4 antibody 11026-1-AP | Download protocol |
| IP protocol for CDK4 antibody 11026-1-AP | Download protocol |
| FC protocol for CDK4 antibody 11026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
品牌介绍
Proteintech Group 由一群当时积极从事 NIH 资助研究的科学家于 2001 年创立。最初的创始人之一和现任首席执行官 Jason Li 博士在公司成立期间是伊利诺伊大学芝加哥分校的免疫学副教授。他谈到 Proteintech 成立背后的原因：“我们抱负的核心是一家由科学家组成的公司；一家为研究人员提供优势的抗体供应商，而不是投资者。”原创产品 – 原创产品Proteintech Group 的部分早期愿景是按照可能的最高标准生产自己的所有产品，并且从不从任何其他供应商处采购单一抗体。这将为研究人员提供许多优势，包括避免由不同公司以不同品牌出售相同抗体的方法——这是行业内出现的一种不受监管的做法1。Proteintech 以 100% 的原创产品为客户提供真诚和正直的品质，您将在本手册中不断发现这些品质。您只能直接从 Proteintech 或通过其批准的经销商之一购买 Proteintech 抗体 - 当您收到抗体并看到小瓶上的 Proteintech 标志时，请知道您拥有真正独一无二的东西。
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