Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells,A431 cells,HeLa cells,HepG2 cells,C2C12 cells,C6 cells |
| Positive IHC detected in | human cervical cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 66 publications below |
| IHC | See 7 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19532-1-AP targets CDK1-Specific inWB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | cell division cycle 2, G1 to S and G2 to M |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | NM_001786 |
| Gene symbol | CDC2 |
| Gene ID (NCBI) | 983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
| IHC protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
| IF protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
