Tested Applications
| Positive WB detected in | HeLa cells,Apoptosised |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Product Information
14311-1-AP targets Caspase 10/p23/17/p12 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 10/p23/17/p12 fusion protein Ag5511 |
| Full Name | caspase 10, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC042844 |
| Gene symbol | CASP10 |
| Gene ID (NCBI) | 843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Caspase10(CASP10)isalsocalledMCH4,isamemberofthepeptidaseC14Afamily,andcontainstwodeath
effector(DED)domains.
WhatisthemolecularweightofCASP10?
ThemolecularweightofCASP10observedonWesternblotsmayvarydependingonwhichofitsisoform,
truncation,orcleavageformsarepresent.Full-lengthCASP10hasapropeptidewith219aminoacidand
6isoformsproducedbyalternativesplicing.
WhatisthetissuespecificityofCASP10?
CASP10isdetectableinmosttissues.However,thereislowerexpressionobservedinbrain,kidney,colon,
prostate,andtestistissue.
WhatisthefunctionofCASP10?
CASP10playsaroleinthecascadeofcaspasesresponsibleforexecutingapoptosis.Itisrecruitedtoboththe
FasandTNFR-1receptorsinaFADD-dependentmanner.CASP10isresponsibleforthecleavageandactivationof
caspases3,4,6,7,8,and9.CASP10canbecleavedbygranzymeBandautocatalyticactivitytogeneratetwo
activesubunitsfromitsinactiveproenzymeform.
WhatistheroleofCASP10indisease?
DefectsinCASP10leadtoautoimmunelymphoproliferativesyndrometype2A(ALPS2A),whichischaracterized
byabnormallymphocyteanddendriticcellhomeostasis,aswellasimmuneregulatorydefects(PMID:16446975).
DefectsinCASP10arealsoacauseoffamilialnon-Hodgkinlymphoma(NHL)andoftenmarkedbyenlargedlymph
nodes,weightlossandfever(PMID:12010812),andgastriccancers(GASC)(PMID:11973654).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
| IHC protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
| IF protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
| IP protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
