ProteinTech/ERK1/2多克隆抗体/150ul/16443-1-AP
商品编号：
16443-1-AP
品牌：
ptglab
市场价：
¥1980.00
美元价：
1188.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue,NIH/3T3 cells,A431 cells,mouse kidney tissue,HEK-293 cells,PC-3 cells,rat brain tissue,rat kidney tissue |
| Positive IHC detected in | human liver tissue, human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 169 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
16443-1-AP targets ERK1/2 inWB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | A. sphaerocephala Krasch, Astragalus membranaceus, chicks, human, mouse, pig, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERK1/2 fusion protein Ag9772 |
| Full Name | mitogen-activated protein kinase 1 |
| Calculated molecular weight | 360 aa, 41 kDa |
| Observed molecular weight | 38-44 kDa |
| GenBank accession number | BC017832 |
| Gene symbol | MAPK1 |
| Gene ID (NCBI) | 5594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 16443-1-AP | Download protocol |
| IHC protocol for ERK1/2 antibody 16443-1-AP | Download protocol |
| IF protocol for ERK1/2 antibody 16443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
品牌介绍
Proteintech Group 由一群当时积极从事 NIH 资助研究的科学家于 2001 年创立。最初的创始人之一和现任首席执行官 Jason Li 博士在公司成立期间是伊利诺伊大学芝加哥分校的免疫学副教授。他谈到 Proteintech 成立背后的原因：“我们抱负的核心是一家由科学家组成的公司；一家为研究人员提供优势的抗体供应商，而不是投资者。”原创产品 – 原创产品Proteintech Group 的部分早期愿景是按照可能的最高标准生产自己的所有产品，并且从不从任何其他供应商处采购单一抗体。这将为研究人员提供许多优势，包括避免由不同公司以不同品牌出售相同抗体的方法——这是行业内出现的一种不受监管的做法1。Proteintech 以 100% 的原创产品为客户提供真诚和正直的品质，您将在本手册中不断发现这些品质。您只能直接从 Proteintech 或通过其批准的经销商之一购买 Proteintech 抗体 - 当您收到抗体并看到小瓶上的 Proteintech 标志时，请知道您拥有真正独一无二的东西。
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