Tested Applications
| Positive WB detected in | RAW264.7, Raji cells,MCF-7 cells,NIH/3T3 cells,HeLa cells,C6 cells,Jurkat cells |
| Positive IHC detected in | human spleen tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 100 publications below |
| IHC | See 14 publications below |
| IF | See 14 publications below |
Product Information
The immunogen of 22915-1-AP is Caspase 1/p20/p10 Fusion Protein expressed in E. coli.
| Tested Reactivity | human,mouse,rat |
| Cited Reactivity | cow, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 1/p20/p10 fusion protein Ag11143 |
| Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
| Calculated molecular weight | 404 aa, 45 kDa |
| Observed molecular weight | 45-47 kDa, 30 kDa, 35 kDa |
| GenBank accession number | BC062327 |
| Gene symbol | CASP1 |
| Gene ID (NCBI) | 834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20..
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
| IHC protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
| IF protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
