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当前位置: 首页 > 产品中心 > peptide > ProteinTech/RB1多克隆抗体/150ul/10048-2-Ig
商品详细ProteinTech/RB1多克隆抗体/150ul/10048-2-Ig
ProteinTech/RB1多克隆抗体/150ul/10048-2-Ig
ProteinTech/RB1多克隆抗体/150ul/10048-2-Ig
商品编号: 10048-2-Ig
品牌: ptglab
市场价: ¥1980.00
美元价: 1188.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Tested Applications

Positive WB detected inJurkat cells,A431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman prostate cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KOSee 3 publications below
WBSee 23 publications below
IHCSee 5 publications below
IFSee 3 publications below

Product Information

10048-2-Ig targets RB1 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.

Tested Reactivityhuman,mouse
Cited Reactivity human, mouse
Host / IsotypeRabbit / IgG
ClassPolyclonal
TypeAntibody
ImmunogenRB1 fusion protein Ag0006
Full Nameretinoblastoma 1
Calculated molecular weight 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC040540
Gene symbolRB1
Gene ID (NCBI)5925
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A purification
Storage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 "Lys-20" trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1"s activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 10048-2-IgDownload protocol
IHC protocol for RB1 antibody 10048-2-IgDownload protocol
IP protocol for RB1 antibody 10048-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
品牌介绍
Proteintech Group 由一群当时积极从事 NIH 资助研究的科学家于 2001 年创立。最初的创始人之一和现任首席执行官 Jason Li 博士在公司成立期间是伊利诺伊大学芝加哥分校的免疫学副教授。他谈到 Proteintech 成立背后的原因:“我们抱负的核心是一家由科学家组成的公司;一家为研究人员提供优势的抗体供应商,而不是投资者。”原创产品 – 原创产品Proteintech Group 的部分早期愿景是按照可能的最高标准生产自己的所有产品,并且从不从任何其他供应商处采购单一抗体。这将为研究人员提供许多优势,包括避免由不同公司以不同品牌出售相同抗体的方法——这是行业内出现的一种不受监管的做法1。Proteintech 以 100% 的原创产品为客户提供真诚和正直的品质,您将在本手册中不断发现这些品质。您只能直接从 Proteintech 或通过其批准的经销商之一购买 Proteintech 抗体 - 当您收到抗体并看到小瓶上的 Proteintech 标志时,请知道您拥有真正独一无二的东西。