Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue,Jurkat cells,mouse brain tissue,mouse liver tissue,mouse testis tissue |
| Positive IHC detected in | human testis tissue, human pancreas tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10022-1-AP targets cIAP1 inWB, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.
| Tested Reactivity | human,mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | cIAP1 fusion protein Ag15203 |
| Full Name | baculoviral IAP repeat-containing 2 |
| Calculated molecular weight | 618 aa, 70 kDa |
| Observed molecular weight | 70 kDa, 55-60 kDa |
| GenBank accession number | BC016174 |
| Gene symbol | BIRC2 |
| Gene ID (NCBI) | 329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| IHC protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| IF protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
