Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells,HL-60 cells,Staurosporine treated Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue,mouse lung tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 299 publications below |
| IHC | See 13 publications below |
| IF | See 4 publications below |
| ELISA | See 1 publications below |
Product Information
10380-1-AP targets Caspase 9/p35/p10 inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Astragalus membranaceus, goat, hamster, hamsters, human, mouse, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 9/p35/p10 fusion protein Ag0404 |
| Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46 kDa, 35 kDa |
| GenBank accession number | BC002452 |
| Gene symbol | CASP9 |
| Gene ID (NCBI) | 842 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IHC protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IF protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IP protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| FC protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
