Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells, RAW264.7,HeLa cells,Raji cells,Jurkat cells,UV treated Hela,Sp2/0 cells |
| Positive IP detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue,human colon tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 128 publications below |
| IHC | See 8 publications below |
| IF | See 2 publications below |
| ELISA | See 1 publications below |
Product Information
13423-1-AP targets Caspase 8/p43/p18 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.
| Tested Reactivity | human,mouse |
| Cited Reactivity | chicken, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 8/p43/p18 fusion protein Ag4214 |
| Full Name | caspase 8, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 538 aa, 62 kDa |
| Observed molecular weight | 53-57 kDa, 32-45 kDa |
| GenBank accession number | BC028223 |
| Gene symbol | CASP8 |
| Gene ID (NCBI) | 841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
| IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
| IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
| IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
