Tested Applications
| Positive WB detected in | Jurkat cells,HEK-293 cells,NIH/3T3 cells, rat kidney tissue,human peripheral blood platelets |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human thyroid cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells,NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 21991-1-AP is PKC Alpha Fusion Protein expressed in E. coli.
| Tested Reactivity | human,mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PKC Alpha fusion protein Ag17275 |
| Full Name | protein kinase C, alpha |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | AK055431 |
| Gene symbol | PRKCA |
| Gene ID (NCBI) | 5578 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PKC Alpha antibody 21991-1-AP | Download protocol |
| IHC protocol for PKC Alpha antibody 21991-1-AP | Download protocol |
| IF protocol for PKC Alpha antibody 21991-1-AP | Download protocol |
| IP protocol for PKC Alpha antibody 21991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
