Tested Applications
| Positive WB detected in | fetal human brain tissue, HEK-293 cells,Neuro-2a cells,ROS1728 cells,human brain tissue,rat brain tissue,mouse brain tissue,pig brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue,human colon cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
60309-1-Ig targets pan Ras inWB, IP, IHC, FC, ELISA applications and shows reactivity with human,mouse,rat,pig samples.
| Tested Reactivity | human,mouse,rat,pig |
| Cited Reactivity | human, swine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | pan Ras fusion protein Ag2700 |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Calculated molecular weight | 188 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC013572 |
| Gene symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| IHC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| IP protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| FC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
