Tested Applications
Positive WB detected in | mouse brain tissue,PC-3 cells,SH-SY5Y cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue,human pancreas cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
Product Information
10889-1-AP targets NPTX2 inWB, IP, IHC, IF, ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NPTX2 fusion protein Ag1326 |
Full Name | neuronal pentraxin II |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC009924 |
Gene symbol | NPTX2 |
Gene ID (NCBI) | 4885 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is secreted protein involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in the Parkinson"s disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)
Protocols
Product Specific Protocols | |
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WB protocol for NPTX2 antibody 10889-1-AP | Download protocol |
IHC protocol for NPTX2 antibody 10889-1-AP | Download protocol |
IP protocol for NPTX2 antibody 10889-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |