Tested Applications
| Positive WB detected in | HeLa cells, soybean whole plant tissue,arabidopsis whole plant tissue,HepG2 cells,ROS1728 cells,pig brain tissue,zebrafish tissue,whole yeast cells,whole Nematode tissue,HEK-293 cells,HSC-T6 cells,PC-12 cells,NIH/3T3 cells,C2C12 cells,SP2/0 cells,rat brain tissue,mouse brain tissue |
| Positive IP detected in | HeLa Cells |
| Positive IF detected in | Ethacrynic acid treated HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB |
| Immunofluorescence (IF) | IF : 1:200-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2758 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
60004-1-Ig targets GAPDH inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human,mouse,rat,yeast, plant samples.
| Tested Reactivity | human,mouse,rat,yeast, plant |
| Cited Reactivity | A. flavus, azalea R. hainanense, beagle, Bovine, canine, carp, chicken, cow, Cynomorium songaricum Rupr, cyprinid |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GAPDH fusion protein Ag0766 |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated molecular weight | 36kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC004109 |
| Gene symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
WhatisthemolecularweightofGAPDH?
ThemolecularweightofGAPDHis36kD.
WhatisGAPDH?
GAPDHstandsforglyceraldehyde-3-phosphatedehydrogenaseandisoftenreferredtoasa“housekeeping”
protein.Itisrecognizedasanimportantenzymeinvolvedinmetabolicpathways,aidingintheglycolytic
productionofATPandpyruvate(PMID:21895736).
WhatisthefunctionofGAPDH?
GAPDHisresponsibleforcatalyzingthephosphorylationofglyceraldehyde-3-phosphateintoD-glycerate-1,3-biphosphate.WhilethisfunctionofGAPDHisconsidereditsprimaryfunction,GAPDHisalsoinvolved,even
critical,inothercellfunctions,someofwhicharestillbeingstudied.RecentstudieshavesuggestedthatGAPDH
isakeyfactorinregulatingapoptosis,translocatingtothenucleusundercertainstressfulconditionsandacting
asasignalingfactorinoxidativestress-inducedapoptosis.OtherstudiesindicatethatGAPDHplaysarolein
transportingvesiclesfromtheendoplasmicreticulumtotheGolgibody(PMIDs:21895736,12829261,25859407,
11035021).
WhereinthecellisGAPDHlocalized?
GAPDHisexpressedprimarilyinthecytosolicandmembraneregions,withminimalexpressioninthenucleus
(PMIDs:21895736,12829261).
IsthereareasontouseanantibodytargetingGAPDHifmyresearchisnotconcernedwithglycolysisornuclear
functions?
Yes!BecauseGAPDHisoftenhighlyexpressedthroughoutdifferenttissuesandcelltypes,GAPDHexpressionis
usefulasacontrolforWesternblotsandRT-PCR.
Whataretheknownprotein-proteininteractionsofGAPDH?
GAPDHinteractswithotherproteinstoregulatenucleartranslocationandGAPDHcatalyticactivity.Someof
theseproteinsincludeTPPP,SIAH1,CHP1,RILPL1/GOSPELandFKBP6(PMIDs:21895736,25859407).
Note:For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| IF protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| FC protocol for GAPDH antibody 60004-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
